Platelet lysate can support the development of a 3D-engineered skin for clinical application.

ABSTRACT: Safety concerns associated with foetal bovine serum (FBS) have restricted its translation into clinics. We hypothesised that platelet lysate (PL) can be utilised as a safe alternative to produce serum-free 3D-engineered skin. PL supported a short-term expansion of fibroblasts, with negligible replication-induced senescence and directed epidermal stratification. PL-expanded fibroblasts were phenotypically separated into three subpopulations of CD90⁺FAP⁺, CD90⁺FAP^- and CD90^-FAP⁺, based on CD90 (reticular marker) and FAP (papillary marker) expression profile. PL drove the expansion of the intermediate CD90⁺ FAP⁺ subpopulation in expense of reticular CD90⁺FAP^-, which may be less fibrot

SUBMITTER: Banakh I 

PROVIDER: S-EPMC9839813 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature