Single-Molecule Study of DNAzyme Reveals Its Intrinsic Conformational Dynamics.

ABSTRACT: DNAzyme is a class of DNA molecules that can perform catalytic functions with high selectivity towards specific metal ions. Due to its potential applications for biosensors and medical therapeutics, DNAzyme has been extensively studied to characterize the relationships between its biochemical properties and functions. Similar to protein enzymes and ribozymes, DNAzymes have been found to undergo conformational changes in a metal-ion-dependent manner for catalysis. Despite the important role the conformation plays in the catalysis process, such structural and dynamic information might not be revealed by conventional approaches. Here, by using the single-molecule fluorescence resonance energy transfer (smFRET) technique, we were able to investigate the detailed conformational dynamics of a ur

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9864658 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature