One-pot encapsulation of lactate dehydrogenase and Fe₃O₄ nanoparticles into a metal-organic framework: A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid.

ABSTRACT: The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes via one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N₂ sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid an

SUBMITTER: Sun X 

PROVIDER: S-EPMC9868447 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature