Characterizing T cell responses to enzymatically modified beta cell neo-epitopes.

ABSTRACT:

Introduction

Previous studies verify the formation of enzymatically post-translationally modified (PTM) self-peptides and their preferred recognition by T cells in subjects with type 1 diabetes (T1D). However, questions remain about the relative prevalence of T cells that recognize PTM self-peptides derived from different antigens, their functional phenotypes, and whether their presence correlates with a specific disease endotype.

Methods

To address this question, we identified a cohort of subjects with T1D who had diverse levels of residual beta cell function. Using previously developed HLA class II tetramer reagents, we enumerated T cells that recognize PTM GAD epitopes in the context of DRB1*04:01 or PTM IA2 epitopes in the context of DQB1*03:02 (DQ8).

Results

Cons

SUBMITTER: Nguyen H 

PROVIDER: S-EPMC9871889 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature