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A Method for Converting HPLC Peak Area from Online Reaction Monitoring to Concentration Using Nonlinear Regression.

ABSTRACT: Online HPLC reaction progress monitoring provides detailed data-rich profiles; however, extracting kinetic information requires ultraviolet-visible response factors to determine concentrations from peak areas. If the reaction's overall mass balance is known and some analytical trend for all relevant species can be recorded, it is possible to estimate the absolute response factors of all species using a system of linear equations. We delineate a method using the Microsoft Solver plug-in to convert time course profiles to reagent concentrations without analytical standards.

SUBMITTER: Deem MC 

PROVIDER: S-EPMC9872085 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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A Method for Converting HPLC Peak Area from Online Reaction Monitoring to Concentration Using Nonlinear Regression.

Deem Madeleine C MC   Hein Jason E JE  

The Journal of organic chemistry 20230110 2

Online HPLC reaction progress monitoring provides detailed data-rich profiles; however, extracting kinetic information requires ultraviolet-visible response factors to determine concentrations from peak areas. If the reaction's overall mass balance is known and some analytical trend for all relevant species can be recorded, it is possible to estimate the absolute response factors of all species using a system of linear equations. We delineate a method using the Microsoft Solver plug-in to conver  ...[more]

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