Activity Hierarchy Measurement to Establish Trace Memory-eligible "Primed" Neurons.

ABSTRACT: Episodic memory is thought to be preferentially encoded by sparsely distributed memory-eligible "primed" neurons in memory-related regions. Based on in vivo calcium imaging on freely behaving mice, we developed an analytical method to determine neuronal activity hierarchy and establish hippocampal primed neurons. Neurons with high activity and memory-associated burst synchronization are identified as primed neurons. When a trace fear memory is being formed or retrieved, the major pattern of the calcium dynamics is predominantly mediated by primed neurons and highly correlated with mouse freezing behaviors. In cilia knockout mice that exhibit severe learning deficits, the percentage of their primed neurons is drastically reduced, and any burst synchronization is strongly suppressed.

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC9881912 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature