Cas9-induced nonhomologous recombination in C. elegans.
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ABSTRACT:
SUBMITTER: Zdraljevic S
PROVIDER: S-EPMC9882370 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Publications
Cas9-induced nonhomologous recombination in <i>C. elegans</i>.
Zdraljevic Stefan S Walter-McNeill Laura L Marquez Heriberto H Kruglyak Leonid L
bioRxiv : the preprint server for biology 20230120
Identification of the genetic basis of phenotypic variation within species remains challenging. In species with low recombination rates, such as <i>Caenorhabditis elegans</i> , genomic regions linked to a phenotype of interest by genetic mapping studies are often large, making it difficult to identify the specific genes and DNA sequence variants that underlie phenotypic differences. Here, we introduce a method that enables researchers to induce targeted recombination in <i>C. elegans</i> with Ca ...[more]
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