Radiosynthesis of 20-[¹⁸F]fluoroarachidonic acid for PET-MR imaging: Biological evaluation in ApoE4-TR mice.

ABSTRACT: Dysreglulated brain arachidonic acid (AA) metabolism is involved in chronic inflammation and is influenced by apolipoprotein E4 (APOE4) genotype, the strongest genetic risk factor of late-onset Alzheimer's disease (AD). Visualization of AA uptake and distribution in the brain can offer insight into neuroinflammation and AD pathogenesis. Here we present a novel synthesis and radiosynthesis of 20-[¹⁸F]fluoroarachidonic acid ([¹⁸F]-FAA) for PET imaging using a convergent route and a one-pot, single-purification radiolabeling procedure, and demonstrate its brain uptake in human ApoE4 targeted replacement (ApoE4-TR) mice. By examining p38 phosphorylation in astrocytes, we found that fluorination of AA at the ω-position did not significantly alter its biochemical role in ce

SUBMITTER: Van Valkenburgh J 

PROVIDER: S-EPMC9888757 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature