Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.

ABSTRACT: Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal γ-tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8- and 16-cell embryos. Their formation was dose-dependently stimulated by m⁶ A, but repressed by m⁵ C modificatio

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC9890236 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature