Phenotypic plasticity of malignant T cells in blood and skin of a Sezary syndrome patient revealed by single cell transcriptomics.

ABSTRACT:

Background

Sézary Syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL). In SS patients, malignant T cells are circulating through the blood and cause erythroderma.

Objective

To compare the transcriptome of single cells in blood and skin samples from a patient with advanced SS.

Methods

We utilized combined single cell RNA and T-cell receptor (TCR) sequencing (scRNA-seq).

Results

We scrutinized the malignant T cells in blood and skin in an unbiased manner without pre-sorting of cells. We observed different phenotypes of the same monoclonal malignant T-cell population, confirmed by TCR sequencing and inferred copy number variation analysis. Malignant T cells present in the circulating blood expressed genes resembling central memory

SUBMITTER: Peiffer L 

PROVIDER: S-EPMC9905421 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature