Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

ABSTRACT:

Background

We investigated the effect of human serum albumin (HSA) on human umbilical cord blood (UCB) CD34⁺ hematopoietic stem/progenitor cells (HSPCs) cultured in vitro and transplanted in vivo.

Methods

Human umbilical cord blood mononuclear cells were obtained by density gradient centrifugation. CD34⁺ cells were then sorted by CD34 conjugated magnetic microbeads. The sorted cells were cultured with or without HSA for 8 days in vitro. After 8 days, all cells were harvested for flow phenotyping and colony formation cell (CFC) experiments. The cells were injected into immunodeficient mice (NOD/Shi-scid/IL2Rγnull, NOG) via intravenous injections. From 4 weeks post-transplantation, flow cytometry was used to calculate human cell chime

SUBMITTER: Hua J 

PROVIDER: S-EPMC9929769 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature