Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions.

ABSTRACT: Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observed

SUBMITTER: Verhulsel M 

PROVIDER: S-EPMC9930731 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature