ALKBH5-mediated m⁶ A demethylation of TIRAP mRNA promotes radiation-induced liver fibrosis and decreases radiosensitivity of hepatocellular carcinoma.

ABSTRACT:

Background

Radiation-induced hepatic stellate cell (HSC) activation promotes radiation-induced liver fibrosis (RILF), a complication for hepatocellular carcinoma (HCC) radiotherapy. The demethylase alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 (ALKBH5) decreases N6-methyladenylate methylation (m⁶ A) modification of RNA, while its role in regulating RILF pathogenesis and HCC radiosensitivity remains unknown.

Methods

Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) were used to screen target genes regulated by ALKBH5. HSC with altered ALKBH5 expression was used to assess irradiation-induced HSC activation and the effect of HSC on recruitment and polarisation of monocytes. Key cytokines in medium from irradiated HSC-ed

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9931500 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature