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Vaccination against SARS-CoV-2 using extracellular blebs derived from spike protein-expressing dendritic cells.

ABSTRACT: COVID-19 has caused significant morbidity and mortality worldwide but also accelerated the clinical use of emerging vaccine formulations. To address the current shortcomings in the prevention and treatment of SARS-CoV-2 infection, this study developed a novel vaccine platform that closely mimics dendritic cells (DCs) in antigen presentation and T-cell stimulation in a cell-free and tunable manner. Genetically engineered DCs that express the SARS-CoV-2 spike protein (S) were chemically converted into extracellular blebs (EBs). The resulting EBs elicited potentially protective humoral immunity in vivo, indicated by the production of antibodies that potently neutralized S-pseudotyped virus, presenting EBs as a promising and safe vaccine.

SUBMITTER: Young Chung J

PROVIDER: S-EPMC9933546 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Vaccination against SARS-CoV-2 using extracellular blebs derived from spike protein-expressing dendritic cells.

Young Chung Jee J Thone Melissa N MN Davies Jenny E JE Gach Johannes S JS Huw Davies D D Forthal Donald N DN Kwon Young Jik YJ

Cellular immunology 20230216

COVID-19 has caused significant morbidity and mortality worldwide but also accelerated the clinical use of emerging vaccine formulations. To address the current shortcomings in the prevention and treatment of SARS-CoV-2 infection, this study developed a novel vaccine platform that closely mimics dendritic cells (DCs) in antigen presentation and T-cell stimulation in a cell-free and tunable manner. Genetically engineered DCs that express the SARS-CoV-2 spike protein (S) were chemically converted ...[more]

PMID: 36822152

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