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Thermo-Programmed Synthetic DNA-Based Receptors.

ABSTRACT: Herein, we present a generalizable and versatile strategy to engineer synthetic DNA ligand-binding devices that can be programmed to load and release a specific ligand at a defined temperature. We do so by re-engineering two model DNA-based receptors: a triplex-forming bivalent DNA-based receptor that recognizes a specific DNA sequence and an ATP-binding aptamer. The temperature at which these receptors load/release their ligands can be finely modulated by controlling the entropy associated with the linker connecting the two ligand-binding domains. The availability of a set of receptors with tunable and reversible temperature dependence allows achieving complex load/release behavior such as sustained ligand release over a wide temperature range. Similar programmable thermo-responsive synthetic ligand-binding devices can be of utility in applications such as drug delivery and production of smart materials.

SUBMITTER: Mariottini D

PROVIDER: S-EPMC9933611 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Thermo-Programmed Synthetic DNA-Based Receptors.

Mariottini Davide D Idili Andrea A Ercolani Gianfranco G Ricci Francesco F

ACS nano 20230123 3

Herein, we present a generalizable and versatile strategy to engineer synthetic DNA ligand-binding devices that can be programmed to load and release a specific ligand at a defined temperature. We do so by re-engineering two model DNA-based receptors: a triplex-forming bivalent DNA-based receptor that recognizes a specific DNA sequence and an ATP-binding aptamer. The temperature at which these receptors load/release their ligands can be finely modulated by controlling the entropy associated with ...[more]

PMID: 36689298

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