Mechanisms of spinophilin-dependent pancreas dysregulation underlying diabesity.

ABSTRACT:

Objective

Spinophilin is an F-actin binding and protein phosphatase 1 (PP1) targeting protein that acts as a scaffold of PP1 to its substrates. Spinophilin knockout (Spino^-/-) mice have decreased fat mass, increased lean mass, and improved glucose tolerance, with no difference in feeding behaviors. While spinophilin is enriched in neurons, its roles in non-neuronal tissues, such as beta cells of the pancreatic islets, are unclear.

Methods & results

We have corroborated and expanded upon previous studies to determine that Spino^-/- mice have decreased weight gain and improved glucose tolerance in two different models of obesity. Using proteomics and immunoblotting-based approaches we identified multiple putative spinophilin interacting proteins isolated f

SUBMITTER: Stickel KC 

PROVIDER: S-EPMC9934546 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature