Ontology highlight
ABSTRACT: Introduction
Post-traumatic coagulopathy (PTC) is a critical pathology in traumatic brain injury (TBI), however, its potential mechanism is not clear. To explore this in peripheral samples, we integrated single cell RNA-sequencing and T cell repertoire (TCR)-sequencing across a cohort of patients with TBI.Methods
Clinical samples from patients with more brain severity demonstrated overexpression of T cell receptor-encoding genes and less TCR diversity.Results
By mapping TCR clonality, we found patients with PTC have less TCR clones, and the TCR clones are mainly distributed in cytotoxic effector CD8+T cell. In addition, the counts of CD8+ T cell and natural killer (NK) cells are associated with the coagulation parameter by WGCNA, and the granzyme and lectin-like receptor profiles are also decreased in the peripheral blood from TBI patients, suggesting that reduced peripheral CD8+ clonality and cytotoxic profiles may be involved in PTC after TBI.Conclusion
Our work systematically revealed the critical immune status in PTC patients at the single-cell level.
SUBMITTER: Zheng P
PROVIDER: S-EPMC9946684 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Zheng Ping P Zhang Ning N Ren Dabin D Yu Cong C Zhao Bin B Bai Qingke Q Zhang Yisong Y Sun Wanju W
Frontiers in immunology 20230208
<h4>Introduction</h4>Post-traumatic coagulopathy (PTC) is a critical pathology in traumatic brain injury (TBI), however, its potential mechanism is not clear. To explore this in peripheral samples, we integrated single cell RNA-sequencing and T cell repertoire (TCR)-sequencing across a cohort of patients with TBI.<h4>Methods</h4>Clinical samples from patients with more brain severity demonstrated overexpression of T cell receptor-encoding genes and less TCR diversity.<h4>Results</h4>By mapping T ...[more]