Mucus secretions from a conditionally reprogrammed primary endocervical cell culture.

ABSTRACT:

Objective

To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.

Design

Experimental.

Setting

University research center.

Animal(s)

Female rhesus macaque (n = 2).

Intervention(s)

None.

Main outcome measure(s)

Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.

Result(s)

We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix.

Conclusion(s)

Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.

SUBMITTER: Park D 

PROVIDER: S-EPMC9947459 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature