Mucus secretions from a conditionally reprogrammed primary endocervical cell culture.

ABSTRACT:

Objective

To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.

Design

Experimental.

Setting

University research center.

Animal(s)

Female rhesus macaque (n = 2).

Intervention(s)

None.

Main outcome measure(s)

Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.

Result(s)

We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of th

SUBMITTER: Park D 

PROVIDER: S-EPMC9947459 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature