Dataset Information

Longitudinal comparison of the developing gut virome in infants and their mothers.

ABSTRACT: The human gut virome and its early life development are poorly understood. Prior studies have captured single-point assessments with the evolution of the infant virome remaining largely unexplored. We performed viral metagenomic sequencing on stool samples collected longitudinally from a cohort of 53 infants from age 2 weeks to 3 years (80.7 billion reads), and from their mothers (9.8 billion reads) to examine and compare viromes. The asymptomatic infant virome consisted of bacteriophages, nonhuman dietary/environmental viruses, and human-host viruses, predominantly picornaviruses. In contrast, human-host viruses were largely absent from the maternal virome. Previously undescribed, sequence-divergent vertebrate viruses were detected in the maternal but not infant virome. As infants aged, the phage component evolved to resemble the maternal virome, but by age 3, the human-host component remained dissimilar from the maternal virome. Thus, early life virome development is determined predominantly by dietary, infectious, and environmental factors rather than direct maternal acquisition.

SUBMITTER: Walters WA

PROVIDER: S-EPMC9950819 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Longitudinal comparison of the developing gut virome in infants and their mothers.

Walters William A WA Granados Andrea C AC Ley Catherine C Federman Scot S Stryke Doug D Santos Yale Y Haggerty Thomas T Sotomayor-Gonzalez Alicia A Servellita Venice V Ley Ruth E RE Parsonnet Julie J Chiu Charles Y CY

Cell host & microbe 20230201 2

The human gut virome and its early life development are poorly understood. Prior studies have captured single-point assessments with the evolution of the infant virome remaining largely unexplored. We performed viral metagenomic sequencing on stool samples collected longitudinally from a cohort of 53 infants from age 2 weeks to 3 years (80.7 billion reads), and from their mothers (9.8 billion reads) to examine and compare viromes. The asymptomatic infant virome consisted of bacteriophages, nonhu ...[more]

PMID: 36758519

Similar Datasets

Project description:BackgroundBreast milk is the nutrient of choice for preterm infants. The World Health Organization has set a target of ensuring by 2030 that 70 % of infants younger than age 6 months are exclusively breastfed. The breastfeeding status of mothers of preterm infants is far below that target. Preterm infants are a special group, and there are many difficulties in and obstacles to adhering to breastfeeding. It is urgent to understand the current situation of breastfeeding among mothers of preterm infants, the change in their breastfeeding behaviors over time, and factors influencing breastfeeding in this population during different postpartum stages.ObjectiveWe aimed to explore potential breastfeeding behavior trajectories and analyze modifiable influencing factors to provide a reference for health care staff to identify risk groups of breastfeeding attrition at an early stage and develop targeted intervention measures.MethodsWe conducted a prospective, single-center, longitudinal study involving a cohort of mothers of preterm infants from July 2023 to May 2024. We administered the Breastfeeding Behavior Questionnaire, Breastfeeding Intention Questionnaire, Breastfeeding Prediction Scale, Breastfeeding Knowledge Questionnaire, and Edinburgh Postnatal Depression Scale. Data were collected at four timepoints: before discharge (T0) and at 6 weeks (T1), 4 months (T2), and 6 months postpartum (T3). A mixed model of latent variable growth was used to fit the trajectory of maternal breastfeeding behavior, and multiple logistic regression analysis was used to detect factors influencing the trajectory of breastfeeding behavior.ResultsThree types of heterogeneity tracks were found: a "high-level rising" group (36.5 %), "medium-level declining" group (26.0 %), and "low-level declining" group (37.5 %). Household income, spouse's educational background, smoking in the spouse, breastfeeding patterns before discharge (T0), perceived breastfeeding control and breastfeeding intention (T1), perceived breastfeeding control, breastfeeding intention, and breastfeeding knowledge (T2), and perceived breastfeeding control, breastfeeding positive attitude, and breastfeeding negative attitude (T3) were predictors of different profiles.ConclusionBreastfeeding is of great importance to promote the growth and development of preterm infants. However, the breastfeeding situation among mothers of preterm infants 6 months after delivery is not encouraging. The rate of exclusive breastfeeding is low and needs to be improved. Clarifying those variables related to the Theory of Planned Behavior, such as breastfeeding positive and negative attitudes, perceived breastfeeding control, breastfeeding knowledge, and postpartum depression score, can provide a theoretical basis for the early identification and screening of mothers at risk of exclusive breastfeeding attrition. Interventions should be delivered at scale to benefit all new mothers and infants.

Dataset Information

Longitudinal comparison of the developing gut virome in infants and their mothers.

Publications

Longitudinal comparison of the developing gut virome in infants and their mothers.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets