Mitochondrial Peroxiredoxin 3 Is Rapidly Oxidized and Hyperoxidized by Fatty Acid Hydroperoxides.

ABSTRACT: Human peroxiredoxin 3 (HsPrx3) is a thiol-based peroxidase responsible for the reduction of most hydrogen peroxide and peroxynitrite formed in mitochondria. Mitochondrial disfunction can lead to membrane lipoperoxidation, resulting in the formation of lipid-bound fatty acid hydroperoxides (_LFA-OOHs) which can be released to become free fatty acid hydroperoxides (_fFA-OOHs). Herein, we report that HsPrx3 is oxidized and hyperoxidized by _fFA-OOHs including those derived from arachidonic acid and eicosapentaenoic acid peroxidation at position 15 with remarkably high rate constants of oxidation (>3.5 × 10⁷ M^-1s^-1) and hyperoxidation (~2 × 10⁷ M^-1s^-1). The endoperoxide-hydroperoxide P

SUBMITTER: Cardozo G 

PROVIDER: S-EPMC9952270 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature