Handling Detection Limits of Multiplex Lateral Flow Immunoassay by Choosing the Order of Binding Zones.

ABSTRACT: Changes in the limits of detection (LODs) for a multiplex lateral flow immunoassay (LFIA) caused by different locations of the binding zone on the test strips were studied. Due to the non-equilibrium conditions of the immune reactions in LFIAs, their analytical parameters are susceptible to the binding constants of antigen-antibody reactions and assay duration. Consequently, the integration of several tests into one multiplex assay can cause a significant worsening of the sensitivity. In this study, we propose a simple methodology for the determination of the best arrangement of binding zones, which takes into account the binding constants for immunoreagents. LFIAs of four mycotoxins, namely, aflatoxin B1, deoxynivalenol, T-2 toxin, and ochratoxin A, were integrated into a multiplex test s

SUBMITTER: Bartosh AV 

PROVIDER: S-EPMC9965744 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature