Exosomes from CD133⁺ human urine-derived stem cells combined adhesive hydrogel facilitate rotator cuff healing by mediating bone marrow mesenchymal stem cells.

ABSTRACT:

Background

The inadequate regeneration of natural tissue (mainly fibrocartilage) between tendon and bone during rotator cuff (RC) repair results in an unsatisfactory quality of RC healing. Cell-free therapy based on stem cell exosomes is a safer and more promising approach for tissue regeneration. Here, we investigated the effect of exosomes from human urine-derived stem cells (USCs) and their subpopulations (CD133⁺USCs) on RC healing.

Methods

USCs were isolated from urine and sorted by flow cytometry to obtain CD133⁺ urine-derived stem cells (CD133⁺ USCs). Urine-derived stem cell exosomes (USC-Exos) and CD133⁺ urine-derived stem cell exosomes (CD133⁺ USC-Exos) were subsequently isolated from the cell supernatant and ide

SUBMITTER: Tong X 

PROVIDER: S-EPMC9984782 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature