Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept.

ABSTRACT:

Objective

To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging.

Approach

Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380-

SUBMITTER: Gomez-Gonzalez E 

PROVIDER: S-EPMC9987604 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature