Genetic and enzymatic characterization of two novel bla_{NDM-36, -37} variants in Escherichia coli strains.

ABSTRACT: The widespread of different NDM variants in clinical Enterobacterales isolates poses a serious public health concern, which requires continuous monitoring. In this study, three E. coli strains carrying two novel bla_NDM variants of bla_{NDM-36, -37} were identified from a patient with refractory urinary tract infection (UTI) in China. We conducted antimicrobial susceptibility testing (AST), enzyme kinetics analysis, conjugation experiment, whole-genome sequencing (WGS), and bioinformatics analysis to characterize the bla_{NDM-36, -37} enzymes and their carrying strains. The bla_{NDM-36, -37} harboring E. coli isolates belonged to ST227, O9:H10 serotype and exhibited intermediate or resistance to all β-lactams tested except aztreonam and aztreonam/avibactam

SUBMITTER: Ma W 

PROVIDER: S-EPMC9998317 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature