Real Time and Spatiotemporal Quantification of pH and H₂O₂ Imbalances with a Multiplex Surface-Enhanced Raman Spectroscopy Nanosensor.

ABSTRACT: Oxidative stress is involved in many aging-related pathological disorders and is the result of defective cellular management of redox reactions. Particularly, hydrogen peroxide (H₂O₂), is a major byproduct and a common oxidative stress biomarker. Monitoring its dynamics and a direct correlation to diseases remains a challenge due to the complexity of redox reactions. Sensitivity and specificity are major drawbacks for H₂O₂ sensors regardless of their readout. Luminiscent boronate-based probes such as 3-mercaptophenylboronic acid (3-MPBA) are emerging as the most effective quantitation tool due to their specificity and sensitivity. Problems associated with these probes are limited intracellular sensing, water solubility, selectivity, and quenching

SUBMITTER: Xiao C 

PROVIDER: S-EPMC9999477 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature