A map of protein dynamics during cell-cycle progression and cell-cycle exit

ABSTRACT: The cell-cycle field has identified the core regulators that drive the cell cycle, but we do not have a clear map of the dynamics of these regulators during cell-cycle progression vs. cell-cycle exit. Here we use single-cell time-lapse microscopy of Cyclin-Dependent Kinase 2 (CDK2) activity followed by end-point immunofluorescence and computational cell synchronization to determine the temporal dynamics of key cell-cycle proteins in asynchronously cycling human cells. We identify several unexpected patterns for core cell-cycle proteins in actively proliferating (CDK2-increasing) vs. spontaneously quiescent (CDK2-low) cells, including Cyclin D1, whose levels we find to be higher in spontaneously quiescent vs. proliferating cells. We also identify proteins whose concentrations steadily increase or decrease the longer cells are in quiescence, suggesting the existence of a continuum of quiescence depths. Our single-cell measurements thus provide a rich resource for the field by characterizing protein dynamics during proliferation vs. quiescence.

SUBMITTER: Mingwei Min 

PROVIDER: S-BSST21 | biostudies-other |

REPOSITORIES: biostudies-other