A perfused iPSC-derived proximal tubule model for predicting drug-induced kidney injury

ABSTRACT: Cell culture insert-based iPSC-derived PT model, which can be maintained in a microphysiological system for at least ten days. We demonstrate the model's ability to predict drug-induced PT injury using polymyxin B, cyclosporin A, and cisplatin, and observe that perfusion distinctly impacts our model’s response to xenobiotics. We observe that the upregulation of metallothioneins that is described in vivo after treatment with these drugs is reliably detected in dynamic, but not static in vitro PT models. Finally, we use our model to alleviate polymyxin-induced nephrotoxicity by supplementing the antioxidant curcumin.

ORGANISM(S): Homo sapiens (human)

SUBMITTER: Eva Dehne 

PROVIDER: S-BSST2218 | biostudies-other |

REPOSITORIES: biostudies-other