Fibroblast diversity within human gut-associated lymphoid tissues

ABSTRACT: The provided data include the scRNA-seq data and flow cytometry metadata of the study entitled "Fibroblast diversity within human gut-associated lymphoid tissues", published in the Journal of Experimental Medicine. Procedure for scRNA-seq: Input: Resection material of patients undergoing surgery for colorectal cancer (healthy controls, samples taken >10cm from tumour site and macroscopically tumour free) or IBD resections from the distal ileum. Samples were processed to obtain single cell suspensions as described by Jørgensen et al. (PMID: 33619391). Cells were sorted on a BD Aria cell sorter. After sorting, the cells were subjected to single cell RNA-sequencing using the 10x Genomics platform. scRNA-seq data is provided as read count matrices as well as FASTQ files that were anonymized using the BAMboozled pipeline (PMID: 34711808). FASTQ files: In short, patient identifiable information such as SNPs, insertions and deletions were replaced with the information of the human reference genome to remove person-identifiable information.

ORGANISM(S): Homo sapiens (human)

SUBMITTER: Urs Mörbe 

PROVIDER: S-BSST2281 | biostudies-other |

REPOSITORIES: biostudies-other