Project description:Temporal analysis of colon carcinoma cell line CC531 response to 4.5 mM butyrate or 3 mM aspirin. Samples taken at 2, 6, 12, 16 and 24 hours. The CC531 cell line has been widely used to study different aspects of tumor growth and metastasis and provides an excellent experimental platform to develop novel antitumor strategies. To characterize the CC531 model at the molecular level, we screened for mutations in genes covering important signal-transduction pathways that are known to play major roles during colon carcinogenesis, the wnt and the ki-ras signaling pathways. We found both a prototypic beta-catenin (Ctnnb1) mutation (Thr(41)Ile) and a ki-ras (G12D) mutation, providing unambiguous evidence for the constitutive activation of these pathways in CC531 cells. We further established comprehensive gene expression profiles of CC531 cells and investigated the molecular response to 2 antitumor drugs, butyrate and aspirin. Using oligonucleotide microarrays, we screened the expression levels of 7,700 genes and identified a total of 398 genes whose expression was significantly changed upon treatment with butyrate. When using aspirin, 121 genes were significantly altered. Interestingly, 36 genes were regulated by both butyrate and aspirin and 35 of them were regulated in the same direction. We found 7 differentially expressed genes, cyclin D1, cyclin E, c-myc, Fosl1, c-fos, Cd44 and follistatin, which are known targets of the beta-catenin and/or the ras pathway. In all cases, butyrate and aspirin reversed the changes in expression normally found in response to active signaling of these oncogenic pathways. Keywords: other

2003-05-19 | GSE424 | GEO

Human colon carcinoma cell line treated with bafilomycin A1

Project description:Analysis of mRNA expression changes in human colon carcinoma cell lines (HCT116 H2B-GFP) after treatment bafilomycin A1. mRNA expression analysis of HCT116 H2B-GFP treated with bafilomycin A1 compared to untreated HCT116 H2B-GFP. Four biological replicates were performed for treated samples.

2014-02-19 | E-GEOD-47836 | biostudies-arrayexpress

A STAT3-inhibitory hairpin decoy oligodeoxynucleotide discriminates between STAT1 and STAT3 and induces death in a human colon carcinoma cell line.

Project description: Not available

| S-EPMC3325846 | biostudies-literature

Colon cancer, cell line, overexpressed CCDC12

Project description:CCDC12 is a newly discovered potential oncogene by our team, and it is proposed to observe the molecules that have interaction with CCDC12 by the technology of proteomic sequencing to map the interaction network of CCDC12. The refined molecular interaction network will help to better observe the role of CCDC12 in cancer for targeted modulation. In this study, we used 4plex iTRAQ technology to sequence proteins from colon cancer cells overexpressing CCDC12, and finally found the differential proteins.

2021-11-11 | PXD029620 | Pride

Murine colon carcinoma cell line (CMT93): control (cont) vs. Lipopolysaccharides (LPS) stimulation

Project description:The gene expression profile of mouse colon carcinoma cell line (CMT93) comparing control untreated CMT93 cells with CMT93 cells stimulated with LPS.

2021-05-30 | GSE175783 | GEO

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