Xenoestrogen Dose-dependent Transcriptomal Changes in MCF-7 Human Breast Cancer Cells

ABSTRACT: Transcriptome analysis of MCF-7 cells exposed for 48 hours to various concentrations of xenoestrogen chemicals. Although biological effects of endocrine disrupting chemicals (EDCs) are often observed at unexpectedly low doses with occasional non-monotonic dose-response characteristics, transcriptome-wide profiles of sensitivities or dose-dependent behaviors of the EDC responsive genes have remained unexplored. Here, we describe expressome analysis for the comprehensive examination of dose-dependent gene responses and its applications to characterize estrogen responsive genes in MCF-7 cells. Transcriptomes of MCF-7 cells exposed to varying concentrations of representative natural and xenobiotic estrogens for 48 hours were determined by microarray and used for computational calculation of in

ORGANISM(S): Homo sapiens

SUBMITTER: Shioda Toshi 

PROVIDER: S-ECPF-GEOD-50705 | biostudies-other |

REPOSITORIES: biostudies-other