STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution.

ABSTRACT: We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 ?m deep penetration into biological tissue.

SUBMITTER: Friedrich M 

PROVIDER: S-EPMC3077687 | biostudies-other | 2011 Apr

REPOSITORIES: biostudies-other