Ontology highlight
ABSTRACT:
SUBMITTER: Read A
PROVIDER: S-EPMC5499874 | biostudies-other | 2017 Jun
REPOSITORIES: biostudies-other
Nucleic acids research 20170601 11
CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool for functional screens. We present here a set of pre-designed human and mouse sgRNA sequences that are optimized for both high on-target potency and low off-target effect. To maximize the chance of target gene inactivation, sgRNAs were curated to target both 5΄ constitutive exons and exons that encode conserved protein domains. We describe here a robust and cost-effective method to construct multiple small sized CRISPR lib ...[more]