Immune cells adapt to confined environments in vivo to optimise nuclear plasticity for migration

ABSTRACT: Cells navigating in complex 3D microenvironments frequently encounter narrow spaces that physically challenge migration. Whilst in vitro studies identified nuclear stiffness as a key rate-limiting factor governing the movement of many cell types through artificial constraints, how cells migrating in vivo respond dynamically to confinement imposed by local tissue architecture, and whether these encounters trigger molecular adaptations, is unclear. Here, we establish an innovative in vivo model for mechanistic analysis of nuclear plasticity as Drosophila immune cells transition into increasingly confined microenvironments. Integrating live in vivo imaging with molecular genetic analyses, we demonstrate how rapid molecular adaptation upon environmental confinement (including fine-tuning of th

SUBMITTER: Helen Weavers 

PROVIDER: S-SCDT-10_1038-S44319-025-00381-0 | biostudies-other |

REPOSITORIES: biostudies-other