Lineage Commitment of Dermal Fibroblast Progenitors is Controlled by Kdm6b-mediated Chromatin Demethylation

ABSTRACT: Dermal Fibroblast Progenitors (DFPs) differentiate into distinct fibroblast lineages during skin development. However, the epigenetic mechanisms that regulate DFP differentiation is not known. Our objective was to use multimodal single-cell approaches, epigenetic assays, and allografting techniques to define DFP state and the mechanism that governs its differentiation potential. Our initial results indicated that the overall transcription profile of DFPs is repressed by H3K27me3 and has inaccessible chromatin at lineage specific genes. Surprisingly, the repressive chromatin profile of DFPs renders them unable to reform skin in allograft assays despite their multipotent potential. We hypothesized that chromatin de-repression was modulated by the H3K27me3 demethylase, Kdm6b/Jmjd3. Dermal fib

SUBMITTER: Quan, M Phan 

PROVIDER: S-SCDT-10_15252-EMBJ_2023113880 | biostudies-other |

REPOSITORIES: biostudies-other