Genome-scale, single cell-type resolution of microRNA activities within a whole plant organ

ABSTRACT: Loaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression and/or accelerated decay of sequence-complementary target transcripts. Despite their importance in development, cell identity-maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non-invasive methodology to examine, at single-cell-type resolution, the AGO-loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root-tip. A dual miRNAome-targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in-dept

SUBMITTER: Dr. Chris, Andrew Brosnan 

PROVIDER: S-SCDT-EMBOJ-2018-100754 | biostudies-other |

REPOSITORIES: biostudies-other