ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival

ABSTRACT: Although lysosomes perform a number of essential cellular functions, damaged lysosomes represent a potential hazard to the cell. Such lysosomes are therefore engulfed by autophagic membranes in the process known as lysophagy, which is initiated by recognition of luminal glycoprotein domains by cytosolic lectins such as Galectin-3. Here we show that, under various conditions that cause injury to the lysosome membrane, components of ESCRT-I, -II and -III are recruited. This recruitment occurs before that of Galectin-3 and the lysophagy machinery. Subunits of the ESCRT-III complex show a particularly prominent recruitment, which depends on the ESCRT-I component TSG101 and the TSG101- and ESCRT-III-binding protein ALIX. Interference with ESCRT recruitment abolishes lysosome repair and causes o

SUBMITTER: Dr. Maja Radulovic 

PROVIDER: S-SCDT-EMBOJ-2018-99753 | biostudies-other |

REPOSITORIES: biostudies-other