Genetic control of pluripotency epigenome determines differentiation bias in embryonic stem cells

ABSTRACT: Genetically diverse pluripotent stem cells display varied, heritable responses to differentiation cues. Here we harnessed these disparities through derivation of mouse embryonic stem cells from the BXD genetic reference panel, along with C57BL/6J (B6) and DBA/2J (D2) parental strains, to identify loci regulating cell state transitions. Upon transition to formative pluripotency, B6 stem cells quickly dissolved naïve networks adopting gene expression modules indicative of neuroectoderm lineages; whereas D2 retained aspects of naïve pluripotency. Spontaneous formation of embryoid bodies identified divergent differentiation where B6 showed a propensity towards neuroectoderm and D2 towards definitive endoderm. Genetic mapping identified major trans-acting loci co-regulating chromatin accessibil

SUBMITTER: Candice Byers 

PROVIDER: S-SCDT-EMBOJ-2021-109445 | biostudies-other |

REPOSITORIES: biostudies-other