Reduced symmetric dimethylation stabilizes vimentin and promotes metastasis in MTAP deficient lung cancer

ABSTRACT: The aggressive nature and poor prognosis of lung cancer led us to explore the mechanisms driving disease progression. Utilizing our invasive cell based model, we identified methylthioadenosine phosphorylase (MTAP) and confirmed its suppressive effects on tumorigenesis and metastasis. Patients with low MTAP expression display worse overall and progression free survival. Mechanistically, accumulation of methylthioadenosine substrate in MTAP deficient cells reduce the level of protein arginine methyltransferase 5 (PRMT5) mediated symmetric dimethylarginine (sDMA) modification on proteins. We identify vimentin as a dimethyl-protein whose dimethylation levels drop in response to MTAP deficiency. The sDMA modification on vimentin reduces its protein abundance but trivially affects its filamentous structure. In MTAP deficient cells, lower sDMA modification prevents ubiquitination mediated vimentin degradation, thereby stabilizing vimentin and contributing to cell invasion. MTAP and PRMT5 negatively correlate with vimentin in lung cancer samples. Taken together, we propose a mechanism for metastasis involving vimentin post-translational regulation.

SUBMITTER: Dr. Wen-Hsin Chang 

PROVIDER: S-SCDT-EMBOR-2021-54265-T | biostudies-other |

REPOSITORIES: biostudies-other