Dataset Information

Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells

ABSTRACT: Cancer relapse upon chemotherapy remains the primary challenge in cancer treatment. By integrating high-resolution CRISPR/Cas9-based evolving lineage tracing with single-cell RNA sequencing in mice, we tracked the evolution of bladder carcinoma in the presence or absence of chemotherapy. We found that a cell population exhibiting an epidermoid-like cell state was able to survive chemotherapy. These epidermoid-like cells left the quiescent state to initiate relapse, generating histologically distinct tumors through different evolutionary routes involving specific transcriptional changes. The plasticity of epidermoid-like cells and alterations in plasticity patterns during evolution have shaped the phenotypes of relapsed tumors. Integrated analyses revealed that Insulin-like growth factor-binding protein 5 was a key co-regulator of aggressive progression and squamous features in bladder carcinoma. Impeding Insulin-like growth factor-binding protein 5 expression suppressed the chemoresistance and malignant behaviors of relapsed tumors. Our study deciphers key events and evolutionary dynamics during the natural progression and recurrence of bladder carcinoma. It offers a viable therapeutic approach to overcome the refractoriness of relapsed bladder carcinoma to conventional chemotherapy.

INSTRUMENT(S): Illumina

ORGANISM(S): Homo Sapiens

SUBMITTER: Shenzhen University, South China Hospital

PROVIDER: PRJNA1435562 | EVA | 2026-03-12

REPOSITORIES: EVA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MP-MGH-U3_somatic_standardized_fixed.vcf.csi	Other
	MP-MGH-U3_somatic_standardized_fixed.vcf.gz	Vcf
	MP-MGH-U3_somatic_standardized_fixed.vcf.gz.csi	Vcf
	UCSD-MGH-U3_somatic_standardized_fixed.vcf.csi	Other
	UCSD-MGH-U3_somatic_standardized_fixed.vcf.gz	Vcf

Items per page:

1 - 5 of 6

Similar Datasets

Project description:We sought to characterize cellular heterogeneity in the human cerebral cortex at a molecular level during cortical neurogenesis. We captured single cells and generated sequencing libraries using the C1TM Single-Cell Auto Prep System (Fluidigm), the SMARTer Ultra Low RNA Kit (Clontech), and the Nextera XT DNA Sample Preparation Kit (Illumina). We performed unbiased clustering of the single cells and further examined transcriptional variation among cell groups interpreted as radial glia. Within this population, the major sources of variation related to cell cycle progression and the stem cell niche from which radial glia were captured. We found that outer subventricular zone radial glia (oRG cells) preferentially express genes related to extracellular matrix formation, migration, and stemness, including TNC, PTPRZ1, FAM107A, HOPX, and LIFR and related this transcriptional state to the position, morphology, and cell behaviors previously used to classify the cell type. Our results suggest that oRG cells maintain the subventricular niche through local production of growth factors, potentiation of growth factor signals by extracellular matrix proteins, and activation of self-renewal pathways, thereby contributing to the developmental and evolutionary expansion of the human neocortex. For study version 2, we have updated this data set to include additional primary cells that we infer to represent microglia, endothelial cells, and immature astrocytes, as well as additional cells from the developing neural retina, and from iPS-cell derived cerebral organoids. The genes distinguishing these cell populations may reveal biological processes supporting the diverse functions of these cell types as well as vulnerabilities of specific cell types in human genetic diseases and in viral infections. For study version 3, we have updated the data set to include additional primary cells, including those published in Nowakowski, et al., Science 2017: "Spatiotemporal Gene Expression Trajectories Reveal Developmental Hierarchies of the Human Cortex" (in press)

Dataset Information

Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets