Transcriptomics,Genomics

Dataset Information

149

Effect of O-GlcNAc transferase deficiency on intestinal epithelial cells in mice


ABSTRACT: O-GlcNAcylation is the modification of serine and threonine residues with beta-N-acetylglucosamine (O-GlcNAc) on intracellular proteins. To investigate the role of protein O-GlcNAcylation on intestinal homeostasis, we generated intestinal epithelial cell (IEC)-specific O-GlcNAc transferase (OGT) knockout in mice. The KO mice developed spontanous intestinal inflammation. To determine the underlying molecular mechanisms, we performed RNA sequencing of ileum and colon epithelial cells of wildtype and IEC-OGT KO mice. Overall design: mRNA profiling of intestinal epithelial cells (IECs) from the ileum and colon of 10-week-old wildtype and IEC-OGT KO male mice.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Hai-Bin Ruan  

PROVIDER: GSE100473 | GEO | 2017-06-27

SECONDARY ACCESSION(S): PRJNA391919

REPOSITORIES: GEO

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Publications

Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.

Zhao Ming M   Xiong Xiwen X   Ren Kaiqun K   Xu Bing B   Cheng Meng M   Sahu Chinmayi C   Wu Kaichun K   Nie Yongzhan Y   Huang Zan Z   Blumberg Richard S RS   Han Xiaonan X   Ruan Hai-Bin HB  

EMBO molecular medicine 20180801 8


Post-translational modifications in intestinal epithelial cells (IECs) allow for precise control in intestinal homeostasis, the breakdown of which may precipitate the pathological damage and inflammation in inflammatory bowel disease. The O-linked β-N-acetylglucosamine (O-GlcNAc) modification on intracellular proteins controls diverse biological processes; however, its roles in intestinal homeostasis are still largely unexplored. Here, we found that levels of protein O-GlcNAcylation and the expr  ...[more]

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