Transcriptomics,Genomics

Dataset Information

160

Linoleic Acid Stimulation of WT Staphylococcus aureus USA300 NRS384


ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA) infections result in more than 200,000 hospitalizations and 10,000 deaths in the United States each year and remain an important medical challenge. A key factor of S. aureus pathogenesis is the production of virulence proteins that are secreted into the extracellular matrix damaging host tissues and forming abscesses that may serve as replicative niches for the bacteria. We recently discovered that host-derived cis-unsaturated fatty acids activate the transcription and translation of EsxA, a protein that plays a central role in abscess formation in clinically relevant MRSA strains. Additionally, we discovered that fatty acid stimulation of EsxA is dependent on fakA, a gene that encodes a protein responsible for the incorporation of exogenous fatty acids into the S. aureus phospholipid membrane. In order to gain a comprehensive understanding of host-fatty-acid-sensing in S. aureus, we performed RNA-Seq analysis on WT Staphylococcus aureus USA300 NRS384, a community-acquired MRSA strain, in the presence and absence of 10μM linoleic acid. Overall design: RNA-Seq analysis was performed on WT Staphylococcus aureus USA300 NRS384 in the presence and absence of 10μM linoleic acid. Each condition was performed in triplicate thereby yielding a total of 6 samples for RNA-Seq analysis.

INSTRUMENT(S): Illumina HiSeq 2500 (Staphylococcus aureus USA300-0114)

SUBMITTER: Mark McCreary  

PROVIDER: GSE101580 | GEO | 2017-11-15

SECONDARY ACCESSION(S): PRJNA397197

REPOSITORIES: GEO

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Publications

Host-derived fatty acids activate type VII secretion in Staphylococcus aureus.

Lopez Michael S MS   Tan Irene S IS   Yan Donghong D   Kang Jing J   McCreary Mark M   Modrusan Zora Z   Austin Cary D CD   Xu Min M   Brown Eric J EJ  

Proceedings of the National Academy of Sciences of the United States of America 20171002 42


The type VII secretion system (T7SS) of Staphylococcus aureus is a multiprotein complex dedicated to the export of several virulence factors during host infection. This virulence pathway plays a key role in promoting bacterial survival and the long-term persistence of staphylococcal abscess communities. The expression of the T7SS is activated by bacterial interaction with host tissues including blood serum, nasal secretions, and pulmonary surfactant. In this work we identify the major stimulator  ...[more]

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