Transcriptomics,Genomics

Dataset Information

39

Global Gene Expression of Methicillin-resistant Staphylococcus aureus USA300 During Human and Mouse Infection


ABSTRACT: Little is known about the expression of methicillin-resistant Staphylococcus aureus (MRSA) genes during infection conditions. Here, we described the transcriptome of the clinical MRSA strain USA300 derived from human cutaneous abscesses, and compared it with USA300 bacteria derived from infected kidneys in a mouse model. Remarkable similarity between the transcriptomes allowed us to identify genes encoding multiple proteases and toxins, and iron- and peptide-transporter molecules, which are upregulated in both infections and are likely important for establishment of infection. We also showed that disruption of the global transcriptional regulators agr and sae prevents in vivo upregulation of many toxins and proteases, protecting mice from lethal infection dose, and hinting at the role of these transcriptional regulators in the pathology of MRSA infection. Overall design: Staphylococcous aureus USA300 were derived from C57Bl/6 mice Mouse kidney abscesses and from human patient cutaneous abscesses. Control wild-type Staphylococcus aureus USA300 samples were taken from 4 hours' culture in TSB. The Mouse, Human, and Control cRNA were Cy5 labeled. A pool of RNA isolated from USA300 bacteria, from various in vitro culture conditions [early log phase (2 h), mid log phase (4 h), post exponential phase (8 h), and stationary phase (20 h) in TSB; mixed at equal ratios] to ensure broad gene expression, was used as reference RNA and was Cy3 labeled.

INSTRUMENT(S): Agilent-022825 S.aureus_020409_4x44K

SUBMITTER: Mark McCreary  

PROVIDER: GSE73821 | GEO | 2015-10-16

SECONDARY ACCESSION(S): PRJNA298051

REPOSITORIES: GEO

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Publications


Little is known about the expression of methicillin-resistant Staphylococcus aureus (MRSA) genes during infection conditions. Here, we described the transcriptome of the clinical MRSA strain USA300 derived from human cutaneous abscesses, and compared it with USA300 bacteria derived from infected kidneys in a mouse model. Remarkable similarity between the transcriptomes allowed us to identify genes encoding multiple proteases and toxins, and iron- and peptide-transporter molecules, which are upre  ...[more]

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