Transcriptomics

Dataset Information

267

Determination of direct binding targets of Quaking RNA binding proteins


ABSTRACT: Identication of RNA molecules directly bound by Quaking proteins in mouse myoblasts and myotubes Overall design: Individual nucleotide resolution crosslinking immunoprecipitation of Qk proteins in either C2C12 myoblasts or 72h differentiated myotubes; control libraries were constructed using mouse anti-HA antibody from C2C12 myoblast cross-linked lysate, other libraries used either mouse anti-PanQk, mouse anti-Qk6, or mouse anti-Qk7 antibody

INSTRUMENT(S): Illumina MiSeq (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: W Samuel Fagg  

PROVIDER: GSE102614 | GEO | 2018-07-11

REPOSITORIES: GEO

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Publications

Autogenous cross-regulation of Quaking mRNA processing and translation balances Quaking functions in splicing and translation.

Fagg W Samuel WS   Liu Naiyou N   Fair Jeffrey Haskell JH   Shiue Lily L   Katzman Sol S   Donohue John Paul JP   Ares Manuel M  

Genes & development 20170901 18


Quaking protein isoforms arise from a single Quaking gene and bind the same RNA motif to regulate splicing, translation, decay, and localization of a large set of RNAs. However, the mechanisms by which Quaking expression is controlled to ensure that appropriate amounts of each isoform are available for such disparate gene expression processes are unknown. Here we explore how levels of two isoforms, nuclear Quaking-5 (Qk5) and cytoplasmic Qk6, are regulated in mouse myoblasts. We found that Qk5 a  ...[more]

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