Transcriptomics,Genomics

Dataset Information

54

Transcriptional reprogramming of human CD4 T cells transitioning from active to memory state


ABSTRACT: The latent reservoir for HIV-1 in resting memory CD4+ T cells is the major barrier to curing HIV-1 infection. Studies of HIV-1 latency have focused on regulation of viral gene expression in cells in which latent infection is established. However, it remains unclear how infection initially becomes latent. Here we described a unique set of properties of CD4+ T cells undergoing effector-to-memory transition including temporary up-regulation of CCR5 expression and rapid down-regulation of cellular gene transcription. These cells allowed completion of steps in the HIV-1 life cycle through integration, but suppressed HIV-1 gene transcription, thus allowing the establishment of latency. CD4+ T cells in this stage were substantially more permissive for HIV-1 latent infection than other CD4+ T cells. Establishment of latent HIV-1 infection in CD4+ T could be inhibited by viral-specific CD8+ T cells, a result with implications for elimination of latent HIV-1 infection by T cell-based vaccines. Overall design: CD4+ T cells from three donors at 5 different treatment time points.

INSTRUMENT(S): [PrimeView] Affymetrix Human Gene Expression Array

SUBMITTER: Liang Shan  

PROVIDER: GSE104275 | GEO | 2017-09-27

SECONDARY ACCESSION(S): PRJNA412219

REPOSITORIES: GEO

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