Genomics

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10x Genonics single-cell RNA-seq of immature (P6) ID4-EGFP mouse spermatogonia & testis cells


ABSTRACT: To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from P6 ID4-EGFP+ spermatogonia (sorted for brightest or dimmest) or unselected testis cells were used for Drop-Seq analysis. The GFP-bright and dim phenotypes exhibit distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the 10x Genomics Chromium (a commercial Drop-Seq variant) to perform single-cell RNA-seq Overall design: Unselected or sorted P6 ID4-EGFP+ spermatogonia (sorted separately as EGFP-bright or EGFP-dim) were used for this study. Data are from a total of 13,094 cells.

INSTRUMENT(S): Illumina HiSeq 3000 (Mus musculus)

SUBMITTER: Brian P. Hermann  

PROVIDER: GSE109049 | GEO | 2018-11-06

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE109049_P6-Id4GFP-10X_filteredmatrix.mtx.gz Other
GSE109049_P6-Id4GFP-10X_filteredmatrixbarcodes.tsv.gz Tabular
GSE109049_P6-Id4GFP-10X_filteredmatrixgenes.tsv.gz Tabular
GSE109049_RAW.tar Raw
filelist.txt Txt
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