Genomics

Dataset Information

0

C1 single-cell RNA-seq of immature (P6) ID4-EGFP mouse spermatoognia


ABSTRACT: To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from P6 ID4-EGFP+ spermatogonia were subdivided into subpopulations that displayed distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq. Overall design: Five replicate preparations of mouse P6 ID4-EGFP+ spermatogonia were used for this study. Data are from a total of 278 cells. Cells were binned into quartiles according to EGFP epifluorescence intensity (Q1 = EGFP-bright, Q4=EGFP-dim, Q2 and Q3 have intermediate EGFP fluorescence) to facilitate comparisons.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Brian P. Hermann  

PROVIDER: GSE108970 | GEO | 2018-11-06

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE108970_P6-Id4GFP-C1_Gematrix.txt.gz Txt
Items per page:
1 - 1 of 1

Similar Datasets

2018-11-06 | GSE108974 | GEO
2018-11-06 | GSE108977 | GEO
2018-11-06 | GSE109049 | GEO
2017-01-01 | S-EPMC5312040 | BioStudies
2018-11-06 | GSE109033 | GEO
2015-01-01 | S-EPMC4342790 | BioStudies
2017-04-24 | GSE83422 | GEO
2016-11-28 | GSE83311 | GEO
| PRJNA429481 | ENA
| PRJNA429263 | ENA