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C1 single-cell RNA-seq of immature (P6) ID4-EGFP mouse spermatoognia

ABSTRACT: To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from P6 ID4-EGFP+ spermatogonia were subdivided into subpopulations that displayed distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE108970 | GEO | 2018/11/06

REPOSITORIES: GEO

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