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Wind-responsive changes in poplar leaves of different developmental stages

ABSTRACT: A developmental series of wind-treated Populus leaf tissue was subjected to array analyses in order to address the issue of age-dependent responsiveness to environmental changes. The following developmental stages were defined for the experiment: Y – “youngest leaf” including the shoot tip = smallest fully enrolled leaf; E – “expanded leaf” = oldest leaf that had not reached full leaf thickness; M – “mature leaf” = 5th leaf below E = has reached full leaf expansion and full leaf thickness; O – “old leaf” = 5th leaf below E. Keywords: transcription profiling

ORGANISM(S): Populus tremula x Populus alba Populus nigra Populus trichocarpa x Populus deltoides Populus euphratica

PROVIDER: GSE11026 | GEO | 2008/04/03

SECONDARY ACCESSION(S): PRJNA107125

REPOSITORIES: GEO

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Wind-responsive changes in poplar leaves of different developmental stages

Project description:A developmental series of wind-treated Populus leaf tissue was subjected to array analyses in order to address the issue of age-dependent responsiveness to environmental changes. The following developmental stages were defined for the experiment: Y – “youngest leaf” including the shoot tip = smallest fully enrolled leaf; E – “expanded leaf” = oldest leaf that had not reached full leaf thickness; M – “mature leaf” = 5th leaf below E = has reached full leaf expansion and full leaf thickness; O – “old leaf” = 5th leaf below E. Keywords: transcription profiling Two-condition experiment, control (K) vs. Wind-treated (W) leaves. Biological replicates: 3 control (1-3), wind-exposed (1-3), independently grown and harvested. One swap replicate per array.

2008-04-03 | E-GEOD-11026 | biostudies-arrayexpress

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Molecular profiling of Arabidopsis leaf growth in LD

Project description:Transcriptome changes during leaf growth and development were profiled. We extracted RNA from Arabidopsis leaf number six from plants growing in stable and controlled experimental conditions at four different growth stages both at the end-of-day (EOD) and end-of-night (EON). The experiment was performed in long-day conditions with optimal soil water content. Four growth stages were selected for analysis: stage 1, with maximum relative area and thickness expansion rates coinciding with leaf emergence; stage 2, maximum area and thickness absolute expansion rates; stage 3, decreasing leaf area and thickness expansion rates, and stage 4, end of leaf area and thickness expansions.

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Gene expression during Arabidopsis flower maturation

Project description:Flower maturation consists of several events that contribute to reproductive success as flowers open, including petal expansion, stamen filament elongation, pollen release, nectary maturation, stigma growth, and gynoecium maturation to support pollen tube growth. The Arabidopsis transcription factors ARF6 (Auxin Response Factor 6) and ARF8 regulate all of these processes, in part by activating jasmonate biosynthesis. Jasmonates in turn activate genes encoding the transcription factors MYB21 and MYB24, which mediate a subset of the processes controlled by ARF6 and ARF8. This experiment was designed to characterize gene expression in flowers before and after they open, and to determine how arf6 arf8 and myb21 myb24 mutation combinations affect these gene expression patterns. Three biological replicates were prepared at each of two developmental stages, stage 12 (oldest closed buds) and stage 13 (youngest open flowers), for three genotypes (Wild type, arf6-2 arf8-3, and myb21-5 myb24-5). For the mutant genotypes, stage 13 flowers do not actually open, so corresponding flowers of equivalent age were chosen based on the position of open flowers in wild-type inflorescences.

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