Project description:We detected DNA methylation of a fibrosarcoma with ESCC sample and the corresponding normal sample to identify the aberrant DNA methylation status in this rare disease.
Project description:We detected the expression of miRNA in a fibrosarcoma with ESCC sample and the corresponding normal esophagus tissue. Then, we identified the differentially expressed miRNAs in this rare disease.
Project description:Fibrosarcoma cell lines were generated from wild-type and Tnfaip8 knockout mice. After stimulation with vehicle, LPA, or PDGF, RNAs were collected from the cells afterwards for RNA-Seq.
Project description:Protein identification in esophageal squamous cell carcinoma(ESCC) was performed,we explored the proteomic landscape in ESCC and attempted to perform clinical-related patient classification based on ESCC proteome.
Project description:Mitochondrial homeostasis is important for cell metabolism, growth, proliferation, and immune responses. The critical regulator for mitochondrial homeostasis, Drp1 and TFAM are frequently abnormal expression in many cancers and is closely implicated in tumorigenesis. Here, we found that Drp1 high expression or TFAM low expression is correlated with poor overall survival of ESCC patients. However, the underling mechanism by Drp1 or TFAM influence tumor progression is largely unknown, especially in esophageal squamous cell carcinoma (ESCC). To investigate the underling mechanisms of Drp1 overexpression or TFAM deficiency-mediated ESCC progression, transcriptome profiling was performed by RNA sequencing analysis in ESCC cells with Drp1 overexpression or TFAM knockdown.
Project description:To define the role of MT1-MMP in the tumor progression, we suppressed its expression in human fibrosarcoma cell line, HT1080, using RNAi technique. The gene expression pattern was then compared betweent the two experimental cell groups with contrasting MT1-MMP expression level. Keywords: other
