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Distinct macrophage populations upon physiological changes in adipose tissue

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

OTHER RELATED OMICS DATASETS IN: PRJNA453187PRJNA453182PRJNA453154PRJNA451512

ORGANISM(S): Mus musculus

PROVIDER: GSE113595 | GEO | 2018/06/04

REPOSITORIES: GEO

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Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue.

Project description:Obesity is characterized by an accumulation of macrophages in adipose, some of which form distinct crown-like structures (CLS) around fat cells. While multiple discrete adipose tissue macrophage (ATM) subsets are thought to exist, their respective effects on adipose tissue, and the transcriptional mechanisms that underlie the functional differences between ATM subsets, are not well understood. We report that obese fat tissue of mice and humans contain multiple distinct populations of ATMs with unique tissue distributions, transcriptomes, chromatin landscapes, and functions. Mouse Ly6c ATMs reside outside of CLS and are adipogenic, while CD9 ATMs reside within CLS, are lipid-laden, and are proinflammatory. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. By contrast, adoptive transfer of CD9 ATMs drives gene expression that is characteristic of obesity. Importantly, human adipose tissue contains similar ATM populations, including lipid-laden CD9 ATMs that increase with body mass. These results provide a higher resolution of the cellular and functional heterogeneity within ATMs and provide a framework within which to develop new immune-directed therapies for the treatment of obesity and related sequela.

| S-EPMC5984532 | biostudies-literature

Distinct macrophage populations upon physiological changes in adipose tissue [ATAC-seq]

Project description:We report that obese fat tissue of mice contain multiple distinct populations of adipose tissue macrophage (ATM) with unique transcriptomes and chromatin landscapes. Mouse Ly6c ATMs express genes that are adipogenic, while CD9 ATMs express pro-inflammatory genes under the control of activating transcription factors. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. By contrast, adoptive transfer of CD9 ATMs drives gene expression that is characteristic of obesity.

2018-06-04 | GSE113583 | GEO

Distinct macrophage populations upon physiological changes in adipose tissue [scRNA-seq]

2018-06-04 | GSE113560 | GEO

Distinct macrophage populations upon physiological changes in adipose tissue [RNA-seq]

2018-06-04 | GSE113594 | GEO

Distinct macrophage populations upon physiological changes in adipose tissue

Project description:Distinct macrophage populations upon physiological changes in adipose tissue

| PRJNA453182 | ENA

Distinct macrophage populations upon physiological changes in adipose tissue [scRNA-seq]

Project description:Distinct macrophage populations upon physiological changes in adipose tissue [scRNA-seq]

| PRJNA451512 | ENA

Distinct macrophage populations upon physiological changes in adipose tissue [RNA-seq]

Project description:Distinct macrophage populations upon physiological changes in adipose tissue [RNA-seq]

| PRJNA453187 | ENA

Distinct macrophage populations upon physiological changes in adipose tissue [ATAC-seq]

Project description:Distinct macrophage populations upon physiological changes in adipose tissue [ATAC-seq]

| PRJNA453154 | ENA

Dynamics of adipose tissue macrophage populations after gastric bypass surgery.

Project description:ObjectiveThis case-control study aimed to analyze the dynamics of macrophage infiltration in subcutaneous adipose tissue following bariatric surgery or conservative treatment of obesity and to clarify whether these features predict the weight loss outcome after the surgery.MethodsSubcutaneous tissue samples taken before and 12 months after laparoscopic Roux-en-Y gastric bypass surgery (n = 39) or conservative (n = 43) treatment for obesity were analyzed. Fat cell size was determined, and with CD68 immunohistochemistry, crown-like structures (CLS) were counted and single macrophages were quantitated.ResultsA major decline in CLS density from 4.1 (SD 3.5) to 1.1 (SD 0.8) per 1000 fat cells (p < 0.000) was found, regardless of the degree of weight loss after the surgery. Surgery had no effect on the fraction of infiltrating single-cell macrophages in subcutaneous adipose tissue. The abundance of these macrophage populations before the intervention did not predict the degree of postsurgery weight loss or suboptimal response to the surgery.ConclusionsThe effect of gastric bypass on adipose tissue inflammatory status associates closely with CLS density even in subjects with suboptimal weight loss. The study suggests that factors related to bypass surgery other than weight loss modify the inflammatory response in adipose tissue.

| S-EPMC10107220 | biostudies-literature

Weight loss independent changes in adipose tissue macrophage and T cell populations after sleeve gastrectomy in mice.

Project description:ObjectiveIn addition to adipocytes, adipose tissue contains large numbers of immune cells. A wide range of evidence links the activity of these cells to regulation of adipocyte and systemic metabolic function. Bariatric surgery improves several aspects of metabolic derangements and at least some of these effects occur in a weight-loss independent manner. We sought to investigate the impact of vertical sleeve gastrectomy (VSG) on adipose immune cell frequencies.MethodsWe analyzed the frequencies of immune cells within distinct adipose tissue depots in obese mice that had VSG or sham surgery with a portion of the latter group pair-fed such that their body mass was matched to the VSG animals.ResultsWe demonstrate that VSG induced a shift in the epididymal adipose tissue leukocyte profile including increased frequencies of CD11c- macrophages, increased frequencies of T cells (CD4+, CD8+, and CD4-/CD8- T cells all increased), but a significantly decreased frequency of adipose tissue dendritic cells (ATDC) that, despite the continued high fat feeding of the VSG group, dropped below control diet levels.ConclusionsThese results indicate that VSG induces substantial changes in the immune populations residing in the adipose depots independent of weight loss.

| S-EPMC5369283 | biostudies-literature

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