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Distinct macrophage populations upon physiological changes in adipose tissue

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

OTHER RELATED OMICS DATASETS IN: PRJNA453187PRJNA453182PRJNA453154PRJNA451512

ORGANISM(S): Mus musculus

PROVIDER: GSE113595 | GEO | 2018/06/04

REPOSITORIES: GEO

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Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue.

Project description:Obesity is characterized by an accumulation of macrophages in adipose, some of which form distinct crown-like structures (CLS) around fat cells. While multiple discrete adipose tissue macrophage (ATM) subsets are thought to exist, their respective effects on adipose tissue, and the transcriptional mechanisms that underlie the functional differences between ATM subsets, are not well understood. We report that obese fat tissue of mice and humans contain multiple distinct populations of ATMs with unique tissue distributions, transcriptomes, chromatin landscapes, and functions. Mouse Ly6c ATMs reside outside of CLS and are adipogenic, while CD9 ATMs reside within CLS, are lipid-laden, and are proinflammatory. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. By contrast, adoptive transfer of CD9 ATMs drives gene expression that is characteristic of obesity. Importantly, human adipose tissue contains similar ATM populations, including lipid-laden CD9 ATMs that increase with body mass. These results provide a higher resolution of the cellular and functional heterogeneity within ATMs and provide a framework within which to develop new immune-directed therapies for the treatment of obesity and related sequela.

| S-EPMC5984532 | biostudies-literature

Distinct macrophage populations upon physiological changes in adipose tissue [scRNA-seq]

Project description:We report that obese fat tissue of mice contain multiple distinct populations of adipose tissue macrophage (ATM) with unique transcriptomes and chromatin landscapes. Mouse Ly6c ATMs express genes that are adipogenic, while CD9 ATMs express pro-inflammatory genes under the control of activating transcription factors. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. By contrast, adoptive transfer of CD9 ATMs drives gene expression that is characteristic of obesity.

2018-06-04 | GSE113560 | GEO

Distinct macrophage populations upon physiological changes in adipose tissue [RNA-seq]

2018-06-04 | GSE113594 | GEO

Distinct macrophage populations upon physiological changes in adipose tissue [ATAC-seq]

2018-06-04 | GSE113583 | GEO

Distinct macrophage populations upon physiological changes in adipose tissue

Project description:Distinct macrophage populations upon physiological changes in adipose tissue

| PRJNA453182 | ENA

Distinct macrophage populations upon physiological changes in adipose tissue [ATAC-seq]

Project description:Distinct macrophage populations upon physiological changes in adipose tissue [ATAC-seq]

| PRJNA453154 | ENA

Distinct macrophage populations upon physiological changes in adipose tissue [RNA-seq]

Project description:Distinct macrophage populations upon physiological changes in adipose tissue [RNA-seq]

| PRJNA453187 | ENA

Distinct macrophage populations upon physiological changes in adipose tissue [scRNA-seq]

Project description:Distinct macrophage populations upon physiological changes in adipose tissue [scRNA-seq]

| PRJNA451512 | ENA

Dynamics of adipose tissue macrophage populations after gastric bypass surgery.

Project description:ObjectiveThis case-control study aimed to analyze the dynamics of macrophage infiltration in subcutaneous adipose tissue following bariatric surgery or conservative treatment of obesity and to clarify whether these features predict the weight loss outcome after the surgery.MethodsSubcutaneous tissue samples taken before and 12 months after laparoscopic Roux-en-Y gastric bypass surgery (n = 39) or conservative (n = 43) treatment for obesity were analyzed. Fat cell size was determined, and with CD68 immunohistochemistry, crown-like structures (CLS) were counted and single macrophages were quantitated.ResultsA major decline in CLS density from 4.1 (SD 3.5) to 1.1 (SD 0.8) per 1000 fat cells (p < 0.000) was found, regardless of the degree of weight loss after the surgery. Surgery had no effect on the fraction of infiltrating single-cell macrophages in subcutaneous adipose tissue. The abundance of these macrophage populations before the intervention did not predict the degree of postsurgery weight loss or suboptimal response to the surgery.ConclusionsThe effect of gastric bypass on adipose tissue inflammatory status associates closely with CLS density even in subjects with suboptimal weight loss. The study suggests that factors related to bypass surgery other than weight loss modify the inflammatory response in adipose tissue.

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Adipose Tissue Macrophage Accrual in Obesity

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-01 | GSE151093 | GEO