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ChIP-seq experiment of H3K4me3 and H3K27me3 in HH30 chicken neural tubes

ABSTRACT: During development, the major class of chromatin regulators is the Polycomb group (PcG) proteins. In this context, they maintain the balance between self-renewal and differentiation. While the role of the Polycomb factor EZH2 has been well described in different developmental contexts, the in vivo activity of EZH1 has long been a puzzle. Here, we show that knocking down EZH1 in chick embryo neural tubes reduces neuronal differentiation without affecting progenitor proliferation or neuronal specification. Transcriptional profiling and functional assays demonstrate that EZH1 mainly acts as a transcriptional activator during neuronal differentiation. Importantly, genes involved in the actin cytoskeleton and cell dynamics that become activated during this process are directly regulated by EZH1. Consistently, depletion of EZH1 in mature neurons leads to morphological alterations and neurite outgrowth defects. Overall, our findings underscore an essential role of EZH1 during early neurogenesis in vivo and highlight the relevance of the dynamic EZH2 to EZH1 switch at the onset of neuronal differentiation.

ORGANISM(S): Gallus gallus

PROVIDER: GSE116042 | GEO | 2026/06/01

REPOSITORIES: GEO

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